PrimePCR™ SYBR® Green Assay: VKORC1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040857

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: VKORC1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: VKORC1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038134
Assay Design:   exonic
Chromosome Location:   16:31102520-31102643question
Amplicon Length:   94
Splice Variants Targeted:   ENST00000313084 ENST00000293350 ENST00000455361 ENST00000433981 ENST00000300851 ENST00000319788 ENST00000354895 ENST00000394975 ENST00000420057 ENST00000394971 ENST00000498155 ENST00000472468 ENST00000584999 ENST00000340830

Gene Information

Vitamin K is essential for blood clotting but must be enzymatically activated. This enzymatically activated form of vitamin K is a reduced form required for the carboxylation of glutamic acid residues in some blood-clotting proteins. The product of this gene encodes the enzyme that is responsible for reducing vitamin K 23-epoxide to the enzymatically activated form. Fatal bleeding can be caused by vitamin K deficiency and by the vitamin K antagonist warfarin and it is the product of this gene that is sensitive to warfarin. In humans mutations in this gene can be associated with deficiencies in vitamin-K-dependent clotting factors and in humans and rats with warfarin resistance. Two pseudogenes have been identified on chromosome 1 and the X chromosome. Two alternatively spliced transcripts encoding different isoforms have been described. [provided by RefSeq Jul 2008]

Gene Symbol:   VKORC1
Gene Name:   vitamin K epoxide reductase complex, subunit 1
Aliases:   EDTP308, FLJ00289, IMAGE3455200, MGC2694, MST134, MST576, VKCFD2, VKOR
RefSeq:   NC_000016.9 NG_011564.1 NT_010393.16
Ensembl:   ENSG00000167397
Entrez:   79001
Chromosome Mapping:   16p11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.900000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download