PrimePCR™ SYBR® Green Assay: NDUFB8, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040853

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038130
Assay Design:   exonic
Chromosome Location:   10:102286188-102286734question
Amplicon Length:   96
Splice Variants Targeted:   ENST00000574608 ENST00000572136 ENST00000576042 ENST00000299166 ENST00000370322 ENST00000370320 ENST00000531258 ENST00000371330 ENST00000337137 ENST00000407381 ENST00000355704 ENST00000406733 ENST00000305031 ENST00000429989 ENST00000372164 ENST00000395139 ENST00000442581 ENST00000428028 ENST00000370033 ENST00000332281 ENST00000514199 ENST00000323146

Gene Information

Description Not Available

Gene Symbol:   NDUFB8
Gene Name:   NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8, 19kDa
Aliases:   ASHI, CI-ASHI
RefSeq:   NC_000010.10 NT_030059.13
Ensembl:   ENSG00000166136
Entrez:   4714
UniGene:   Hs.523215
Chromosome Mapping:   10q24.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.970000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download