PrimePCR™ SYBR® Green Assay: IFI27L1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040852

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: IFI27L1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: IFI27L1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038129
Assay Design:   exonic
Chromosome Location:   14:94568883-94568972question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000279028 ENST00000289422 ENST00000361474 ENST00000340391 ENST00000289409 ENST00000358522 ENST00000541337 ENST00000545385 ENST00000394770 ENST00000396435 ENST00000375368 ENST00000568956 ENST00000264607 ENST00000576655 ENST00000555523 ENST00000393115 ENST00000556381 ENST00000553664 ENST00000557218 ENST00000554544 ENST00000557066 ENST00000554562 ENST00000327044 ENST00000307142 ENST00000429713 ENST00000434046 ENST00000504178 ENST00000367033 ENST00000318602 ENST00000250572 ENST00000312053 ENST

Gene Information

Description Not Available

Gene Symbol:   IFI27L1
Gene Name:   interferon, alpha-inducible protein 27-like 1
Aliases:   FAM14B, ISG12C
RefSeq:   NC_000014.8 NT_026437.12
Ensembl:   ENSG00000165948
Entrez:   122509
Chromosome Mapping:   14q32.12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.720000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download