PrimePCR™ SYBR® Green Assay: BRI3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040845

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: BRI3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: BRI3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038122
Assay Design:   exonic
Chromosome Location:   7:97911695-97920442question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000289672 ENST00000273261 ENST00000383703 ENST00000328933 ENST00000458238 ENST00000290607 ENST00000357450 ENST00000380034 ENST00000380027 ENST00000396847 ENST00000412903 ENST00000419850 ENST00000396855 ENST00000396853 ENST00000305836 ENST00000398145 ENST00000402105 ENST00000336873 ENST00000398141 ENST00000454703 ENST00000515030 ENST00000503407 ENST00000281455 ENST00000283875 ENST00000547303 ENST00000551116 ENST00000346473 ENST00000552740 ENST00000547526 ENST00000361661 ENST00000438251 ENST

Gene Information

Description Not Available

Gene Symbol:   BRI3
Gene Name:   brain protein I3
Aliases:   I3
RefSeq:   NC_000007.13 NG_013090.1 NT_007933.15
Ensembl:   ENSG00000164713
Entrez:   25798
Chromosome Mapping:   7q21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 35.340000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download