PrimePCR™ SYBR® Green Assay: DISC1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040835

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038112
Assay Design:   exonic
Chromosome Location:   1:231830381-231830542question
Amplicon Length:   132
Splice Variants Targeted:   ENST00000508064 ENST00000311573 ENST00000368340 ENST00000347088 ENST00000361831 ENST00000355499 ENST00000404643 ENST00000359205 ENST00000407221 ENST00000295566 ENST00000368330 ENST00000368339 ENST00000535662 ENST00000317586 ENST00000539444 ENST00000602281 ENST00000366633 ENST00000366636 ENST00000366637 ENST00000439617 ENST00000535983 ENST00000537876

Gene Information

This gene encodes a protein with multiple coiled coil motifs which is located in the nucleus cytoplasm and mitochondria. The protein is involved in neurite outgrowth and cortical development through its interaction with other proteins. This gene is disrupted in a t(1;11)(q42.1;q14.3) translocation which segregates with schizophrenia and related psychiatric disorders in a large Scottish family. Alternate transcriptional splice variants encoding different isoforms have been characterized. [provided by RefSeq Jul 2008]

Gene Symbol:   DISC1
Gene Name:   disrupted in schizophrenia 1
Aliases:   C1orf136, FLJ13381, FLJ21640, FLJ25311, FLJ41105, KIAA0457, SCZD9
RefSeq:   NC_000001.10 NT_167186.1 NG_011681.1
Ensembl:   ENSG00000162946
Entrez:   27185
UniGene:   Hs.13318
Chromosome Mapping:   1q42.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.740000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download