PrimePCR™ SYBR® Green Assay: EDA, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040822

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: EDA, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: EDA, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038099
Assay Design:   exonic
Chromosome Location:   X:68836324-68836543question
Amplicon Length:   190
Splice Variants Targeted:   ENST00000525810 ENST00000527388 ENST00000374553 ENST00000374552 ENST00000524573 ENST00000338901 ENST00000377246 ENST00000349847

Gene Information

The protein encoded by this gene is a type II membrane protein that can be cleaved by furin to produce a secreted form. The encoded protein which belongs to the tumor necrosis factor family acts as a homotrimer and may be involved in cell-cell signaling during the development of ectodermal organs. Defects in this gene are a cause of ectodermal dysplasia anhidrotic which is also known as X-linked hypohidrotic ectodermal dysplasia. Several transcript variants encoding many different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   EDA
Gene Name:   ectodysplasin A
Aliases:   ED1, ED1-A1, ED1-A2, EDA1, EDA2, HED, ODT1, STHAGX1, XHED, XLHED
RefSeq:   NC_000023.10 NG_021246.1 NT_011669.17 NG_009809.1
Ensembl:   ENSG00000158813
Entrez:   1896
Chromosome Mapping:   Xq12-q13.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.790000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download