PrimePCR™ SYBR® Green Assay: KCNMA1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040818

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: KCNMA1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: KCNMA1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038095
Assay Design:   exonic
Chromosome Location:   10:79397049-79397231question
Amplicon Length:   153
Splice Variants Targeted:   ENST00000244333 ENST00000595358 ENST00000323084 ENST00000397916 ENST00000496391 ENST00000397912 ENST00000354783 ENST00000372437 ENST00000372408 ENST00000457953 ENST00000372421 ENST00000404771 ENST00000372440 ENST00000372443 ENST00000372403 ENST00000286628 ENST00000286627 ENST00000406533 ENST00000354353 ENST00000404857 ENST00000564985 ENST00000569623

Gene Information

MaxiK channels are large conductance voltage and calcium-sensitive potassium channels which are fundamental to the control of smooth muscle tone and neuronal excitability. MaxiK channels can be formed by 2 subunits: the pore-forming alpha subunit which is the product of this gene and the modulatory beta subunit. Intracellular calcium regulates the physical association between the alpha and beta subunits. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq Jul 2008]

Gene Symbol:   KCNMA1
Gene Name:   potassium large conductance calcium-activated channel, subfamily M, alpha member 1
Aliases:   BKTM, DKFZp686K1437, KCa1.1, MGC71881, MaxiK, SAKCA, SLO, SLO-ALPHA, SLO1, bA205K10.1, mSLO1
RefSeq:   NC_000010.10 NG_012270.1 NT_030059.13
Ensembl:   ENSG00000156113
Entrez:   3778
Chromosome Mapping:   10q22.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.270000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download