PrimePCR™ SYBR® Green Assay: RBMX, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040802

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038079
Assay Design:   exonic
Chromosome Location:   X:135961181-135961491question
Amplicon Length:   85
Splice Variants Targeted:   ENST00000321792 ENST00000399794 ENST00000424776 ENST00000431446 ENST00000419968 ENST00000320676 ENST00000562646 ENST00000570135 ENST00000598507

Gene Information

This gene belongs to the RBMY gene family which includes candidate Y chromosome spermatogenesis genes. This gene an active X chromosome homolog of the Y chromosome RBMY gene is widely expressed whereas the RBMY gene evolved a male-specific function in spermatogenesis. Pseudogenes of this gene found on chromosomes 1 4 9 11 and 6 were likely derived by retrotransposition from the original gene. Alternatively spliced transcript variants encoding different isoforms have been identified. A snoRNA gene (SNORD61) is found in one of its introns. [provided by RefSeq Sep 2009]

Gene Symbol:   RBMX
Gene Name:   RNA binding motif protein, X-linked
RefSeq:   NC_000023.10 NG_012918.1 NT_011786.16
Ensembl:   ENSG00000147274
Entrez:   27316
UniGene:   Hs.380118
Chromosome Mapping:   Xq26.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.970000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download