PrimePCR™ SYBR® Green Assay: SLC26A1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040797

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038074
Assay Design:   exonic
Chromosome Location:   4:985068-985183question
Amplicon Length:   86
Splice Variants Targeted:   ENST00000426955 ENST00000273794 ENST00000332700 ENST00000373625 ENST00000396017 ENST00000398520 ENST00000361661 ENST00000398516 ENST00000586305 ENST00000454404 ENST00000293062 ENST00000587738 ENST00000445061 ENST00000348968 ENST00000457607 ENST00000446256

Gene Information

This gene is a member of a family of sulfate/anion transporter genes. Family members are well conserved in their genomic (number and size of exons) and protein (aa length among species) structures but have markedly different tissue expression patterns. This gene is primarily expressed in the liver pancreas and brain. Three splice variants that encode different isoforms have been identified. [provided by RefSeq Jul 2008]

Gene Symbol:   SLC26A1
Gene Name:   solute carrier family 26 (sulfate transporter), member 1
Aliases:   EDM4, SAT-1, SAT1
RefSeq:   NC_000004.11 NG_008103.1 NT_037622.5
Ensembl:   ENSG00000145217
Entrez:   10861
UniGene:   Hs.658244
Chromosome Mapping:   4p16.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.710000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download