PrimePCR™ SYBR® Green Assay: GEMIN7, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040789

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038066
Assay Design:   exonic
Chromosome Location:   19:45593581-45593716question
Amplicon Length:   106
Splice Variants Targeted:   ENST00000378623 ENST00000358511 ENST00000453409 ENST00000412892 ENST00000335211 ENST00000295591 ENST00000357836 ENST00000336751 ENST00000591607 ENST00000591747 ENST00000270257 ENST00000391951 ENST00000377111 ENST00000377110 ENST00000579423 ENST00000580823

Gene Information

The protein encoded by this gene is a component of the core SMN complex which is required for pre-mRNA splicing in the nucleus. The encoded protein is found in the nucleoplasm in nuclear "gems" (Gemini of Cajal bodies) and in the cytoplasm. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   GEMIN7
Gene Name:   gem (nuclear organelle) associated protein 7
Aliases:   SIP3
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000142252
Entrez:   79760
UniGene:   Hs.466919
Chromosome Mapping:   19q13.32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.830000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download