PrimePCR™ SYBR® Green Assay: CGREF1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040780

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CGREF1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CGREF1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038057
Assay Design:   exonic
Chromosome Location:   2:27325228-27325413question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000327669 ENST00000298532 ENST00000402550 ENST00000402394 ENST00000405600 ENST00000312734 ENST00000404694 ENST00000452318 ENST00000260595 ENST00000530505 ENST00000293874 ENST00000409527 ENST00000409439 ENST00000422307 ENST00000321878 ENST00000439574 ENST00000026218 ENST00000470411 ENST00000035307 ENST00000495645 ENST00000541206 ENST00000409345

Gene Information

Description Not Available

Gene Symbol:   CGREF1
Gene Name:   cell growth regulator with EF-hand domain 1
Aliases:   CGR11
RefSeq:   NC_000002.11 NG_012199.1 NT_022184.15
Ensembl:   ENSG00000138028
Entrez:   10669
Chromosome Mapping:   2p23.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.340000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download