PrimePCR™ SYBR® Green Assay: BLOC1S1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040772

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: BLOC1S1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: BLOC1S1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038049
Assay Design:   exonic
Chromosome Location:   12:56112931-56113299question
Amplicon Length:   63
Splice Variants Targeted:   ENST00000549424 ENST00000550412 ENST00000257899 ENST00000548556 ENST00000551926 ENST00000547076 ENST00000549147 ENST00000548925

Gene Information

BLOC1S1 is a component of the ubiquitously expressed BLOC1 multisubunit protein complex. BLOC1 is required for normal biogenesis of specialized organelles of the endosomal-lysosomal system such as melanosomes and platelet dense granules (Starcevic and Dell'Angelica 2004 [PubMed 15102850]).[supplied by OMIM Mar 2008]

Gene Symbol:   BLOC1S1
Gene Name:   biogenesis of lysosomal organelles complex-1, subunit 1
Aliases:   BLOS1, FLJ39337, FLJ97089, GCN5L1, MGC87455, MICoA, RT14
RefSeq:   NC_000012.11 NG_008606.1 NG_012343.1 NT_029419.12
Ensembl:   ENSG00000135441
Entrez:   2647
Chromosome Mapping:   12q13-q14

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 35.670000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download