PrimePCR™ SYBR® Green Assay: MKRN1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040766

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: MKRN1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MKRN1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038043
Assay Design:   exonic
Chromosome Location:   7:140171700-140171801question
Amplicon Length:   72
Splice Variants Targeted:   ENST00000255977 ENST00000474576 ENST00000480552 ENST00000443720 ENST00000471104 ENST00000467513 ENST00000494939 ENST00000473444 ENST00000437223

Gene Information

The Makorin ring finger protein-1 gene (MKRN1) is a highly transcribed intron-containing source for a family of intronless mammalian genes encoding a novel class of zinc finger proteins. Phylogenetic analyses indicate that the MKRN1 gene is the ancestral founder of this gene family (Gray et al. 2000 [PubMed 10843807]).[supplied by OMIM Mar 2008]

Gene Symbol:   MKRN1
Gene Name:   makorin ring finger protein 1
Aliases:   FLJ21334, RNF61
RefSeq:   NC_000007.13 NT_007914.15
Ensembl:   ENSG00000133606
Entrez:   23608
Chromosome Mapping:   7q34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.780000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download