PrimePCR™ SYBR® Green Assay: NSUN5, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040751

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: NSUN5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: NSUN5, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038028
Assay Design:   exonic
Chromosome Location:   7:72717232-72717433question
Amplicon Length:   172
Splice Variants Targeted:   ENST00000320736 ENST00000420699 ENST00000461153 ENST00000331428 ENST00000447459 ENST00000380898 ENST00000541430 ENST00000397389 ENST00000373095 ENST00000373084 ENST00000428206 ENST00000252594 ENST00000438747 ENST00000310326 ENST00000319914 ENST00000219478 ENST00000456329 ENST00000233055 ENST00000575600 ENST00000570494 ENST00000573847 ENST00000577167

Gene Information

This gene encodes a member of the evolutionarily conserved NOL1/NOP2/Sun domain family. The encoded protein may function as a DNA methyltransferase in the nucleus. This gene is deleted in Williams syndrome a multisystem developmental disorder caused by the deletion of contiguous genes at 7q11.23. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq Dec 2009]

Gene Symbol:   NSUN5
Gene Name:   NOP2/Sun domain family, member 5
Aliases:   FLJ10267, MGC986, NOL1, NOL1R, NSUN5A, WBSCR20, WBSCR20A, p120
RefSeq:   NC_000007.13 NT_007933.15
Ensembl:   ENSG00000130305
Entrez:   55695
Chromosome Mapping:   7q11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 36.580000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download