PrimePCR™ SYBR® Green Assay: ECSIT, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040750

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ECSIT, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ECSIT, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038027
Assay Design:   exonic
Chromosome Location:   19:11618301-11618530question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000308020 ENST00000193403 ENST00000394419 ENST00000438964 ENST00000376839 ENST00000538545 ENST00000355272 ENST00000345016 ENST00000370225 ENST00000536513 ENST00000535881 ENST00000270517 ENST00000588998 ENST00000252440 ENST00000417981 ENST00000591104 ENST00000592312 ENST00000585318 ENST00000297488 ENST00000381861 ENST00000262102 ENST00000519263 ENST00000381869 ENST00000544260 ENST00000400046 ENST00000356501 ENST00000359635 ENST00000355349 ENST00000490337 ENST00000471742 ENST00000475456 ENST

Gene Information

Description Not Available

Gene Symbol:   ECSIT
Gene Name:   ECSIT homolog (Drosophila)
Aliases:   SITPEC
RefSeq:   NC_000019.9 NT_011295.11
Ensembl:   ENSG00000130159
Entrez:   51295
Chromosome Mapping:   19p13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 37.290000
Efficiency 90

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download