PrimePCR™ SYBR® Green Assay: APOBEC3A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040745

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038022
Assay Design:   exonic
Chromosome Location:   22:39358615-39358755question
Amplicon Length:   111
Splice Variants Targeted:   ENST00000319921 ENST00000552744 ENST00000457153 ENST00000261205 ENST00000393240 ENST00000249116 ENST00000402255 ENST00000491143 ENST00000262095 ENST00000216218 ENST00000411695 ENST00000551589 ENST00000372592 ENST00000308700 ENST00000404699 ENST00000291294 ENST00000261523 ENST00000376351 ENST00000589078 ENST00000262623 ENST00000261980 ENST00000360319 ENST00000369850 ENST00000344736 ENST00000422373 ENST00000256062 ENST00000551659 ENST00000552618 ENST00000539277 ENST00000381224 ENST00000307808 ENST

Gene Information

This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster thought to result from gene duplication on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. The protein encoded by this gene lacks the zinc binding activity of other family members. The protein plays a role in immunity by restricting transmission of foreign DNA such as viruses. One mechanism of foreign DNA restriction is deamination of foreign double-stranded DNA cytidines to uridines which leads to DNA degradation. However other mechanisms are also thought to be involved as anti-viral effect is not dependent on deaminase activity. One allele of this gene results from the deletion of approximately 29.5 kb of sequence between this gene APOBEC3A and the adjacent gene APOBEC3B. The breakpoints of the deletion are within the two genes so the deletion allele is predicted to have the promoter and coding region of APOBEC3A but the 3' UTR of APOBEC3B. [provided by RefSeq Jul 2010]

Gene Symbol:   APOBEC3A
Gene Name:   apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3A
Aliases:   ARP3, PHRBN, bK150C2.1
RefSeq:   NC_000022.10 NT_011520.12 NW_003315972.1
Ensembl:   ENSG00000128383
Entrez:   200315
UniGene:   Hs.731690
Chromosome Mapping:   22q13.1-q13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.994900
y-intercept 36.560000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download