PrimePCR™ SYBR® Green Assay: PSG8, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040733

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038010
Assay Design:   exonic
Chromosome Location:   19:43262256-43262382question
Amplicon Length:   97
Splice Variants Targeted:   ENST00000327495 ENST00000595140 ENST00000404209 ENST00000406636 ENST00000306511 ENST00000187910 ENST00000402603 ENST00000292125 ENST00000594375 ENST00000403486 ENST00000320078 ENST00000306322 ENST00000401740 ENST00000406487 ENST00000599812 ENST00000405312 ENST00000244295 ENST00000596907 ENST00000599391 ENST00000599371 ENST00000451895 ENST00000593948 ENST00000270077 ENST00000244293

Gene Information

The human pregnancy-specific glycoproteins (PSGs) are a group of molecules that are mainly produced by the placental syncytiotrophoblasts during pregnancy. PSGs comprise a subgroup of the carcinoembryonic antigen (CEA) family which belongs to the immunoglobulin superfamily. For additional general information about the PSG gene family see PSG1 (MIM 176390).[supplied by OMIM Oct 2009]

Gene Symbol:   PSG8
Gene Name:   pregnancy specific beta-1-glycoprotein 8
Aliases:   DKFZp781L10202, PSG1
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000124467
Entrez:   440533
UniGene:   Hs.466843
Chromosome Mapping:   19q13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.030000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download