PrimePCR™ SYBR® Green Assay: LAMA4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040711

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037988
Assay Design:   exonic
Chromosome Location:   6:112575337-112575483question
Amplicon Length:   117
Splice Variants Targeted:   ENST00000230538 ENST00000522006 ENST00000389463 ENST00000424408 ENST00000521398 ENST00000519932 ENST00000431543 ENST00000243219 ENST00000521690 ENST00000368638 ENST00000453937 ENST00000455073 ENST00000584825 ENST00000579568 ENST00000578058 ENST00000578144 ENST00000579010 ENST00000582024 ENST00000580457 ENST00000583235 ENST00000579840 ENST00000584877 ENST00000584945 ENST00000578470

Gene Information

Laminins a family of extracellular matrix glycoproteins are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion differentiation migration signaling neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha beta and gamma (formerly A B1 and B2 respectively) and they form a cruciform structure consisting of 3 short arms each formed by a different chain and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown but some of the chains have been shown to differ with respect to their tissue distribution presumably reflecting diverse functions in vivo. This gene encodes the alpha chain isoform laminin alpha 4. The domain structure of alpha 4 is similar to that of alpha 3 both of which resemble truncated versions of alpha 1 and alpha 2 in that approximately 1200 residues at the N-terminus (domains IV V and VI) have been lost. Laminin alpha 4 contains the C-terminal G domain which distinguishes all alpha chains from the beta and gamma chains. The RNA analysis from adult and fetal tissues revealed developmental regulation of expression however the exact function of laminin alpha 4 is not known. Tissue-specific utilization of alternative polyA-signal has been described in literature. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq Aug 2011]

Gene Symbol:   LAMA4
Gene Name:   laminin, alpha 4
Aliases:   DKFZp686D23145, LAMA3, LAMA4*-1
RefSeq:   NC_000006.11 NT_025741.15 NG_008209.1
Ensembl:   ENSG00000112769
Entrez:   3910
UniGene:   Hs.654572
Chromosome Mapping:   6q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.280000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download