PrimePCR™ SYBR® Green Assay: DHRS11, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040702

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: DHRS11, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: DHRS11, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037979
Assay Design:   exonic
Chromosome Location:   17:34955352-34955457question
Amplicon Length:   76
Splice Variants Targeted:   ENST00000583023 ENST00000257570 ENST00000292579 ENST00000417550 ENST00000378230 ENST00000355186 ENST00000426804 ENST00000357029 ENST00000434111 ENST00000331505 ENST00000433039 ENST00000394071 ENST00000286523 ENST00000251312 ENST00000590554 ENST00000491046 ENST00000585376 ENST00000602840 ENST00000356056 ENST00000564571 ENST00000566885 ENST00000424560 ENST00000444904 ENST00000568873

Gene Information

Description Not Available

Gene Symbol:   DHRS11
Gene Name:   dehydrogenase/reductase (SDR family) member 11
Aliases:   ARPG836, FLJ39232, MGC4172, SDR24C1
RefSeq:   NC_000017.10 NT_010783.15 NW_003315949.1
Ensembl:   ENSG00000108272
Entrez:   79154
Chromosome Mapping:   17q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.490000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download