PrimePCR™ SYBR® Green Assay: SSBP1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040694

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SSBP1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SSBP1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037971
Assay Design:   exonic
Chromosome Location:   7:141443367-141443721question
Amplicon Length:   124
Splice Variants Targeted:   ENST00000265304 ENST00000498107 ENST00000467681 ENST00000465582 ENST00000463093 ENST00000484178 ENST00000481508 ENST00000570667 ENST00000571430 ENST00000576311 ENST00000575059 ENST00000571709 ENST00000574199 ENST00000576834

Gene Information

SSBP1 is a housekeeping gene involved in mitochondrial biogenesis (Tiranti et al. 1995 [PubMed 7789991]). It is also a subunit of a single-stranded DNA (ssDNA)-binding complex involved in the maintenance of genome stability (Huang et al. 2009) [PubMed 19683501].[supplied by OMIM Feb 2010]

Gene Symbol:   SSBP1
Gene Name:   single-stranded DNA binding protein 1
Aliases:   Mt-SSB, SOSS-B1, SSBP, mtSSB
RefSeq:   NC_000007.13 NT_007914.15 NW_003315922.1
Ensembl:   ENSG00000106028
Entrez:   6742
Chromosome Mapping:   7q34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.130000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download