PrimePCR™ SYBR® Green Assay: CAPN3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040672

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037949
Assay Design:   exonic
Chromosome Location:   15:42703108-42703486question
Amplicon Length:   62
Splice Variants Targeted:   ENST00000370355 ENST00000238831 ENST00000357568 ENST00000349748 ENST00000397163 ENST00000397200 ENST00000569827 ENST00000337571 ENST00000569136 ENST00000561817 ENST00000397204 ENST00000564503 ENST00000356316 ENST00000318023 ENST00000236959 ENST00000368820 ENST00000377075

Gene Information

Calpain a heterodimer consisting of a large and a small subunit is a major intracellular protease although its function has not been well established. This gene encodes a muscle-specific member of the calpain large subunit family that specifically binds to titin. Mutations in this gene are associated with limb-girdle muscular dystrophies type 2A. Alternate promoters and alternative splicing result in multiple transcript variants encoding different isoforms and some variants are ubiquitously expressed. [provided by RefSeq Jul 2008]

Gene Symbol:   CAPN3
Gene Name:   calpain 3, (p94)
Aliases:   CANP3, CANPL3, LGMD2, LGMD2A, MGC10767, MGC11121, MGC14344, MGC4403, nCL-1, p94
RefSeq:   NC_000015.9 NG_008660.1 NT_010194.17
Ensembl:   ENSG00000092529
Entrez:   825
UniGene:   Hs.143261
Chromosome Mapping:   15q15.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.995600
y-intercept 36.450000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download