PrimePCR™ SYBR® Green Assay: EPDR1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040665

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: EPDR1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: EPDR1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037942
Assay Design:   exonic
Chromosome Location:   7:37989802-37989914question
Amplicon Length:   83
Splice Variants Targeted:   ENST00000559325 ENST00000425345 ENST00000423717 ENST00000199448 ENST00000476620

Gene Information

The protein encoded by this gene is a type II transmembrane protein that is similar to two families of cell adhesion molecules the protocadherins and ependymins. This protein may play a role in calcium-dependent cell adhesion. This protein is glycosylated and the orthologous mouse protein is localized to the lysosome. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 8. [provided by RefSeq Aug 2011]

Gene Symbol:   EPDR1
Gene Name:   ependymin related protein 1 (zebrafish)
Aliases:   EPDR, MERP-1, MERP1, UCC1
RefSeq:   NC_000007.13 NT_007819.17
Ensembl:   ENSG00000086289
Entrez:   54749
Chromosome Mapping:   7p14.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998200
y-intercept 35.970000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download