PrimePCR™ SYBR® Green Assay: SLC9A3R2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040652

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SLC9A3R2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SLC9A3R2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037929
Assay Design:   exonic
Chromosome Location:   16:2087539-2087857question
Amplicon Length:   63
Splice Variants Targeted:   ENST00000479808 ENST00000216254 ENST00000396512 ENST00000508053 ENST00000262464 ENST00000424542 ENST00000432365 ENST00000563587 ENST00000561844 ENST00000565855 ENST00000566198 ENST00000373249 ENST00000317257 ENST00000258672 ENST00000354234 ENST00000373387 ENST00000435213 ENST00000314904 ENST00000594524

Gene Information

This gene encodes a member of the NHERF family of PDZ scaffolding proteins. These proteins mediate many cellular processes by binding to and regulating the membrane expression and protein-protein interactions of membrane receptors and transport proteins. The encoded protein plays a role in intestinal sodium absorption by regulating the activity of the sodium/hydrogen exchanger 3 and may also regulate the cystic fibrosis transmembrane regulator (CFTR) ion channel. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq Nov 2011]

Gene Symbol:   SLC9A3R2
Gene Name:   solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 2
Aliases:   E3KARP, MGC104639, NHE3RF2, NHERF-2, NHERF2, OCTS2, SIP-1, SIP1, TKA-1
RefSeq:   NC_000016.9 NG_008412.1 NT_010393.16
Ensembl:   ENSG00000065054
Entrez:   9351
Chromosome Mapping:   16p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.610000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download