PrimePCR™ SYBR® Green Assay: THOC3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040648

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037925
Assay Design:   exonic
Chromosome Location:   5:175386926-175387114question
Amplicon Length:   159
Splice Variants Targeted:   ENST00000427611 ENST00000434415 ENST00000265097 ENST00000505969 ENST00000514861 ENST00000589553

Gene Information

TEX1 is part of the TREX (transcription/export) complex which includes THO2 (MIM 300395) HPR1 (MIM 606930) ALY (MIM 604171) and UAP56 (MIM 142560).[supplied by OMIM Nov 2010]

Gene Symbol:   THOC3
Gene Name:   THO complex 3
Aliases:   MGC5469, TEX1
RefSeq:   NC_000005.9 NT_023133.13
Ensembl:   ENSG00000051596
Entrez:   84321
UniGene:   Hs.484227
Chromosome Mapping:   5q35.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.660000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download