PrimePCR™ SYBR® Green Assay: THOC3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040648

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: THOC3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: THOC3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037925
Assay Design:   exonic
Chromosome Location:   5:175386926-175387114question
Amplicon Length:   159
Splice Variants Targeted:   ENST00000427611 ENST00000434415 ENST00000265097 ENST00000505969 ENST00000514861 ENST00000589553

Gene Information

TEX1 is part of the TREX (transcription/export) complex which includes THO2 (MIM 300395) HPR1 (MIM 606930) ALY (MIM 604171) and UAP56 (MIM 142560).[supplied by OMIM Nov 2010]

Gene Symbol:   THOC3
Gene Name:   THO complex 3
Aliases:   MGC5469, TEX1
RefSeq:   NC_000005.9 NT_023133.13
Ensembl:   ENSG00000051596
Entrez:   84321
Chromosome Mapping:   5q35.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.660000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download