PrimePCR™ SYBR® Green Assay: HFE, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040640

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: HFE, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: HFE, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037917
Assay Design:   exonic
Chromosome Location:   6:26092948-26093070question
Amplicon Length:   93
Splice Variants Targeted:   ENST00000397022 ENST00000353147 ENST00000349999 ENST00000317896 ENST00000357618 ENST00000470149 ENST00000336625 ENST00000461397 ENST00000488199 ENST00000309234 ENST00000360319 ENST00000369850 ENST00000422373 ENST00000369856 ENST00000346618 ENST00000535432 ENST00000596447 ENST00000597475 ENST00000600520 ENST00000594381

Gene Information

The protein encoded by this gene is a membrane protein that is similar to MHC class I-type proteins and associates with beta2-microglobulin (beta2M). It is thought that this protein functions to regulate iron absorption by regulating the interaction of the transferrin receptor with transferrin. The iron storage disorder hereditary haemochromatosis is a recessive genetic disorder that results from defects in this gene. At least nine alternatively spliced variants have been described for this gene. Additional variants have been found but their full-length nature has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   HFE
Gene Name:   hemochromatosis
Aliases:   HFE1, HH, HLA-H, MGC103790, MVCD7, dJ221C16.10.1
RefSeq:   NC_000006.11 NG_001335.1 NG_008720.1 NT_007592.15
Ensembl:   ENSG00000010704
Entrez:   3077
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.550000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download