PrimePCR™ SYBR® Green Assay: LEPROT, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040595

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: LEPROT, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: LEPROT, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037872
Assay Design:   exonic
Chromosome Location:   1:65900457-65900598question
Amplicon Length:   112
Splice Variants Targeted:   ENST00000371065

Gene Information

LEPROT is associated with the Golgi complex and endosomes and has a role in cell surface expression of growth hormone receptor (GHR; MIM 600946) and leptin receptor (OBR or LEPR; MIM 601007) thereby altering receptor-mediated cell signaling (Couturier et al. 2007 [PubMed 18042720]; Touvier et al. 2009 [PubMed 19907080]).[supplied by OMIM Jul 2010]

Gene Symbol:   LEPROT
Gene Name:   leptin receptor overlapping transcript
Aliases:   FLJ37482, FLJ90360, LEPR, OB-RGRP, OBRGRP, VPS55
RefSeq:   NG_015831.1 NC_000001.10 NT_032977.9
Ensembl:   ENSG00000213625
Entrez:   54741
Chromosome Mapping:   1p31.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.650000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download