This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0040595
PrimePCR™ PreAmp for SYBR® Green Assay: LEPROT, Human
PrimePCR™ Template for SYBR® Green Assay: LEPROT, Human
LEPROT is associated with the Golgi complex and endosomes and has a role in cell surface expression of growth hormone receptor (GHR; MIM 600946) and leptin receptor (OBR or LEPR; MIM 601007) thereby altering receptor-mediated cell signaling (Couturier et al. 2007 [PubMed 18042720]; Touvier et al. 2009 [PubMed 19907080]).[supplied by OMIM Jul 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.