This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0040561
PrimePCR™ PreAmp for SYBR® Green Assay: AP2S1, Human
PrimePCR™ Template for SYBR® Green Assay: AP2S1, Human
One of two major clathrin-associated adaptor complexes AP-2 is a heterotetramer which is associated with the plasma membrane. This complex is composed of two large chains a medium chain and a small chain. This gene encodes the small chain of this complex. Alternative splicing has been observed in this gene and results in two known transcripts. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.