PrimePCR™ SYBR® Green Assay: HTR3D, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040554

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: HTR3D, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: HTR3D, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037831
Assay Design:   exonic
Chromosome Location:   3:183756828-183756932question
Amplicon Length:   75
Splice Variants Targeted:   ENST00000334128 ENST00000382489 ENST00000453435

Gene Information

The protein encoded this gene belongs to the ligand-gated ion channel receptor superfamily. This gene encodes subunit D of the type 3 receptor for 5-hydroxytryptamine (serotonin) a biogenic hormone that functions as a neurotransmitter a mitogen and a hormone. This hormone has been linked to neuropsychiatric disorders including anxiety depression and migraine. Serotonin receptors causes fast and depolarizing responses in neurons following activation. The genes encoding subunits C D and E of this type 3 receptor form a cluster on chromosome 3. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2009]

Gene Symbol:   HTR3D
Gene Name:   5-hydroxytryptamine (serotonin) receptor 3 family member D
Aliases:   5HT3D, MGC119636, MGC119637
RefSeq:   NC_000003.11 NT_005612.16 NG_012750.1
Ensembl:   ENSG00000186090
Entrez:   200909
Chromosome Mapping:   3q27.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.870000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download