PrimePCR™ SYBR® Green Assay: ECHDC1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040407

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037684
Assay Design:   exonic
Chromosome Location:   6:127648165-127651966question
Amplicon Length:   107
Splice Variants Targeted:   ENST00000454859 ENST00000531967 ENST00000474289 ENST00000528402 ENST00000454591 ENST00000368291 ENST00000430841 ENST00000368289 ENST00000436638 ENST00000525745 ENST00000534442 ENST00000531582 ENST00000309620

Gene Information

Description Not Available

Gene Symbol:   ECHDC1
Gene Name:   enoyl CoA hydratase domain containing 1
Aliases:   DKFZp762M1110, FLJ40827, dJ351K20.2
RefSeq:   NC_000006.11 NT_025741.15
Ensembl:   ENSG00000093144
Entrez:   55862
UniGene:   Hs.486410
Chromosome Mapping:   6q22.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.840000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download