PrimePCR™ SYBR® Green Assay: UGT1A9, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040399

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037676
Assay Design:   exonic
Chromosome Location:   2:234581177-234581322question
Amplicon Length:   116
Splice Variants Targeted:   ENST00000354728

Gene Information

This gene encodes a UDP-glucuronosyltransferase an enzyme of the glucuronidation pathway that transforms small lipophilic molecules such as steroids bilirubin hormones and drugs into water-soluble excretable metabolites. This gene is part of a complex locus that encodes several UDP-glucuronosyltransferases. The locus includes thirteen unique alternate first exons followed by four common exons. Four of the alternate first exons are considered pseudogenes. Each of the remaining nine 5' exons may be spliced to the four common exons resulting in nine proteins with different N-termini and identical C-termini. Each first exon encodes the substrate binding site and is regulated by its own promoter. The enzyme encoded by this gene is active on phenols. [provided by RefSeq Jul 2008]

Gene Symbol:   UGT1A9
Gene Name:   UDP glucuronosyltransferase 1 family, polypeptide A9
Aliases:   HLUGP4, LUGP4, UDPGT, UDPGT 1-9, UGT1-09, UGT1AI
RefSeq:   NG_009254.1 NC_000002.11 NG_002601.2 NT_005120.16
Ensembl:   ENSG00000241119
Entrez:   54600
UniGene:   Not Available
Chromosome Mapping:   2q37

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 35.720000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download