PrimePCR™ SYBR® Green Assay: IL18BP, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040390

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: IL18BP, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: IL18BP, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037667
Assay Design:   exonic
Chromosome Location:   11:71711484-71711595question
Amplicon Length:   82
Splice Variants Targeted:   ENST00000393703 ENST00000497194 ENST00000393705 ENST00000337131 ENST00000531053 ENST00000393707 ENST00000404792 ENST00000260049

Gene Information

The protein encoded by this gene functions as an inhibitor of the proinflammatory cytokine IL18. It binds IL18 prevents the binding of IL18 to its receptor and thus inhibits IL18-induced IFN-gamma production resulting in reduced T-helper type 1 immune responses. This protein is constitutively expressed and secreted in mononuclear cells. Elevated level of this protein is detected in the intestinal tissues of patients with Crohn's disease. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq Feb 2011]

Gene Symbol:   IL18BP
Gene Name:   interleukin 18 binding protein
Aliases:   IL18BPa
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000137496
Entrez:   10068
Chromosome Mapping:   11q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.130000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download