This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0040381
PrimePCR™ PreAmp for SYBR® Green Assay: AGMO, Human
PrimePCR™ Template for SYBR® Green Assay: AGMO, Human
Alkylglycerol monooxygenase (AGMO; EC 18.104.22.168) cleaves the O-alkyl bonds of ether lipids which are essential components of brain membranes and function in cell signaling and other critical biologic processes. The reaction catalyzed by AGMO is dependent on tetrahydrobiopterin a metabolite structurally related to the vitamins folic acid and riboflavin (summary by Watschinger et al. 2010 [PubMed 20643956]).[supplied by OMIM Feb 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.