PrimePCR™ SYBR® Green Assay: ST7L, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0040316

List Price:    $174.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037593
Assay Design:   exonic
Chromosome Location:   1:113126636-113134160question
Amplicon Length:   96
Splice Variants Targeted:   ENST00000358039 ENST00000360743 ENST00000369669 ENST00000490067 ENST00000343210 ENST00000369666 ENST00000418497 ENST00000369664 ENST00000544629 ENST00000369668 ENST00000538187 ENST00000543570

Gene Information

This gene was identified by its similarity to the ST7 tumor suppressor gene found in the chromosome 7q31 region. This gene is clustered in a tail-to-tail manner with the WNT2B gene in a chromosomal region known to be deleted and rearranged in a variety of cancers. Several transcript variants encoding many different isoforms have been described but some have not been fully characterized. [provided by RefSeq Feb 2011]

Gene Symbol:   ST7L
Gene Name:   suppression of tumorigenicity 7 like
Aliases:   FAM4B, FLJ11657, FLJ20284, ST7R, STLR
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000007341
Entrez:   54879
Chromosome Mapping:   1p13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.970000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download