This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0040312
PrimePCR™ PreAmp for SYBR® Green Assay: RAD23A, Human
PrimePCR™ Template for SYBR® Green Assay: RAD23A, Human
The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23 a protein involved in nucleotide excision repair (NER). This protein was shown to interact with and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG) which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain which was reported to interact with 26S proteasome as well as with ubiquitin protein ligase E6AP and thus suggests that this protein may be involved in the ubiquitin mediated proteolytic pathway in cells. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.