PrimePCR™ SYBR® Green Assay: UBL7, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040302

List Price:    $134.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037579
Assay Design:   exonic
Chromosome Location:   15:74748922-74751042question
Amplicon Length:   78
Splice Variants Targeted:   ENST00000567435 ENST00000565335 ENST00000395081 ENST00000361351 ENST00000564488 ENST00000565130 ENST00000563081

Gene Information

Description Not Available

Gene Symbol:   UBL7
Gene Name:   ubiquitin-like 7 (bone marrow stromal cell-derived)
Aliases:   BMSC-UbP, MGC14421, TCBA1
RefSeq:   NC_000015.9 NT_010194.17
Ensembl:   ENSG00000138629
Entrez:   84993
UniGene:   Hs.334713
Chromosome Mapping:   15q24.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.030000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download