PrimePCR™ SYBR® Green Assay: TSPY1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040285

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TSPY1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TSPY1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037562
Assay Design:   exonic
Chromosome Location:   Y:9307181-9307281question
Amplicon Length:   71
Splice Variants Targeted:   ENST00000320701 ENST00000383042 ENST00000426950 ENST00000383008 ENST00000287721 ENST00000383000 ENST00000383005 ENST00000330628 ENST00000457222 ENST00000424594 ENST00000440483 ENST00000427871 ENST00000451548 ENST00000423647 ENST00000440215 ENST00000446779 ENST00000428845 ENST00000444056 ENST00000429039

Gene Information

The protein encoded by this gene is found only in testicular tissue and may be involved in spermatogenesis. Approximately 35 copies of this gene are present in humans but only a single nonfunctional orthologous gene is found in mouse. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Oct 2010]

Gene Symbol:   TSPY1
Gene Name:   testis specific protein, Y-linked 1
Aliases:   CT78, DYS14, TSPY, pJA923
RefSeq:   NC_000024.9 NG_027958.1 NT_011878.9
Ensembl:   ENSG00000258992
Entrez:   7258
Chromosome Mapping:   Yp11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.750000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download