PrimePCR™ SYBR® Green Assay: ARPC4-TTLL3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040254

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ARPC4-TTLL3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ARPC4-TTLL3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037531
Assay Design:   exonic
Chromosome Location:   3:9876420-9876556question
Amplicon Length:   107
Splice Variants Targeted:   ENST00000394357 ENST00000572933 ENST00000393319 ENST00000568883 ENST00000373606 ENST00000397150 ENST00000397152 ENST00000373605 ENST00000397151 ENST00000397156 ENST00000392011 ENST00000409212 ENST00000344528 ENST00000508471 ENST00000290974 ENST00000511071 ENST00000515312 ENST00000374599 ENST00000374597 ENST00000252336 ENST00000266736 ENST00000290015 ENST00000393461 ENST00000426488 ENST00000394983 ENST00000300849 ENST00000539836 ENST00000535577 ENST00000538906 ENST00000397256 ENST00000426895 ENST

Gene Information

This locus represents naturally occurring read-through transcription between the neighboring ARPC4 (actin related protein 2/3 complex subunit 4) and TTLL3 (tubulin tyrosine ligase-like family member 3) genes. The read-through transcript encodes a fusion protein that shares sequence identity with each individual gene product. [provided by RefSeq Nov 2010]

Gene Symbol:   ARPC4-TTLL3
Gene Name:   ARPC4-TTLL3 readthrough
Aliases:   FLJ14048
RefSeq:   Not Available
Ensembl:   ENSG00000250151
Entrez:   100526693
Chromosome Mapping:   3p

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.240000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download