PrimePCR™ SYBR® Green Assay: XXbac-BPG181M17.5, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040248

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037525
Assay Design:   exonic
Chromosome Location:   6:32906642-32906737question
Amplicon Length:   66
Splice Variants Targeted:   ENST00000439344 ENST00000412948 ENST00000449417 ENST00000413617 ENST00000395312 ENST00000435056 ENST00000552299 ENST00000548611 ENST00000548183 ENST00000317419 ENST00000317089 ENST00000447454 ENST00000440078 ENST00000449679 ENST00000548290 ENST00000547478 ENST00000551481 ENST00000456428 ENST00000450897 ENST00000448590 ENST00000418759 ENST00000424822 ENST00000430099 ENST00000428864 ENST00000383231 ENST00000415090 ENST00000552389 ENST00000549152 ENST00000546855 ENST00000401088 ENST00000401089 ENST

Gene Information

Description Not Available

Gene Symbol:   XXbac-BPG181M17.5
Gene Name:   Major histocompatibility complex, class II, DM beta
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSG00000248993
UniGene:   Not Available
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 36.100000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download