PrimePCR™ SYBR® Green Assay: ATP5J2-PTCD1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040246

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ATP5J2-PTCD1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ATP5J2-PTCD1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037523
Assay Design:   exonic
Chromosome Location:   7:99022650-99022761question
Amplicon Length:   82
Splice Variants Targeted:   ENST00000292478 ENST00000555673 ENST00000413834 ENST00000422202 ENST00000265909 ENST00000311620 ENST00000260643 ENST00000406567 ENST00000337907 ENST00000377464 ENST00000476556 ENST00000361711 ENST00000343939 ENST00000407464 ENST00000407598 ENST00000443632 ENST00000310544 ENST00000514112 ENST00000413580 ENST00000330843 ENST00000281961

Gene Information

Description Not Available

Gene Symbol:   ATP5J2-PTCD1
Gene Name:   ATP5J2-PTCD1 readthrough
Aliases:   FLJ56092
RefSeq:   Not Available
Ensembl:   ENSG00000248919
Entrez:   100526740
Chromosome Mapping:   7q

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.240000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download