PrimePCR™ SYBR® Green Assay: CTAGE8, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040241

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CTAGE8, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CTAGE8, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037518
Assay Design:   exonic
Chromosome Location:   7:143966180-143966295question
Amplicon Length:   86
Splice Variants Targeted:   ENST00000314099 ENST00000553728 ENST00000554392 ENST00000555716 ENST00000341749 ENST00000557038 ENST00000396165 ENST00000280083 ENST00000348007 ENST00000553352 ENST00000341502 ENST00000396158 ENST00000486333 ENST00000487179 ENST00000315874 ENST00000354440 ENST00000450189 ENST00000542579 ENST00000399850 ENST00000427450

Gene Information

Description Not Available

Gene Symbol:   CTAGE8
Gene Name:   CTAGE family, member 8
Aliases:   FLJ77176
RefSeq:   Not Available
Ensembl:   ENSG00000244693
Entrez:   100142659
Chromosome Mapping:   7q

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 34.830000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download