PrimePCR™ SYBR® Green Assay: XXbac-BPG116M5.17, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040238

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: XXbac-BPG116M5.17, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: XXbac-BPG116M5.17, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037515
Assay Design:   exonic
Chromosome Location:   6:31915163-31915285question
Amplicon Length:   93
Splice Variants Targeted:   ENST00000417261 ENST00000427888 ENST00000399981 ENST00000419920 ENST00000396593 ENST00000425368 ENST00000556679 ENST00000307741 ENST00000586677 ENST00000456570 ENST00000477310 ENST00000375443 ENST00000375455 ENST00000426239 ENST00000419411 ENST00000518586 ENST00000335171 ENST00000521644 ENST00000375662 ENST00000424727 ENST00000436692 ENST00000433503 ENST00000455591

Gene Information

Description Not Available

Gene Symbol:   XXbac-BPG116M5.17
Gene Name:   Complement factor B; Uncharacterized protein; Uncharacterized protein; cDNA FLJ55673, highly similar to Complement factor B
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSG00000244255
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.600000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download