PrimePCR™ SYBR® Green Assay: CT45A5, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040222

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037499
Assay Design:   exonic
Chromosome Location:   X:134948015-134948152question
Amplicon Length:   108
Splice Variants Targeted:   ENST00000370741 ENST00000370736 ENST00000495729 ENST00000370734 ENST00000443882 ENST00000434966 ENST00000494421 ENST00000420087 ENST00000370724 ENST00000491480 ENST00000448053 ENST00000472834 ENST00000594565 ENST00000596640 ENST00000598716 ENST00000595754 ENST00000594117 ENST00000601753

Gene Information

This gene represents one of a cluster of six similar genes located on the q arm of chromosome X. The genes in this cluster encode members of the cancer/testis (CT) family of antigens and are distinct from other CT antigens. These antigens are thought to be novel therapeutic targets for human cancers. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 5. [provided by RefSeq May 2010]

Gene Symbol:   CT45A5
Gene Name:   cancer/testis antigen family 45, member A5
Aliases:   CT45.5, CT455
RefSeq:   NC_000023.10 NG_016551.1 NT_011786.16
Ensembl:   ENSG00000242284
Entrez:   441521
UniGene:   Hs.535081
Chromosome Mapping:   Xq26.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.010000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download