PrimePCR™ SYBR® Green Assay: EGFL8, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040217

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037494
Assay Design:   exonic
Chromosome Location:   6:32133915-32134025question
Amplicon Length:   81
Splice Variants Targeted:   ENST00000418522 ENST00000433364 ENST00000414111 ENST00000549741 ENST00000455889 ENST00000452290 ENST00000423079 ENST00000467460 ENST00000550232 ENST00000447331 ENST00000399648 ENST00000422193 ENST00000443511 ENST00000418252 ENST00000421005 ENST00000547891 ENST00000436155 ENST00000450526 ENST00000454755 ENST00000547558 ENST00000452189 ENST00000413733 ENST00000411747 ENST00000437001 ENST00000581472 ENST00000333845 ENST00000395512 ENST00000432129

Gene Information

Description Not Available

Gene Symbol:   EGFL8
Gene Name:   EGF-like-domain, multiple 8
Aliases:   C6orf8, FLJ44493, FLJ52513, FLJ75166, FLJ78536, FLJ97375, MGC44938, MGC59719, NG3
RefSeq:   NC_000006.11 NT_007592.15 NT_113891.2 NT_167244.1 NT_167246.1 NT_167247.1 NT_167248.1 NT_167249.1
Ensembl:   ENSG00000241404
Entrez:   80864
UniGene:   Hs.731888
Chromosome Mapping:   6p21.32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.090000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download