PrimePCR™ SYBR® Green Assay: RPP21, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040216

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: RPP21, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RPP21, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037493
Assay Design:   exonic
Chromosome Location:   6:30314220-30314310question
Amplicon Length:   61
Splice Variants Targeted:   ENST00000552257 ENST00000425575 ENST00000376642 ENST00000430900 ENST00000415583 ENST00000550581 ENST00000412659 ENST00000416977 ENST00000439624 ENST00000427637 ENST00000550618 ENST00000551862 ENST00000456870 ENST00000434282 ENST00000422320 ENST00000422720 ENST00000430819 ENST00000414187 ENST00000452262 ENST00000439797 ENST00000513556 ENST00000433076 ENST00000442966 ENST00000428040 ENST00000436442 ENST00000552950 ENST00000552136 ENST00000455025 ENST00000424616 ENST00000411784 ENST00000437470 ENST

Gene Information

RPP21 is a protein subunit of nuclear ribonuclease P which processes the 5-prime leader sequence of precursor tRNAs (Jarrous et al. 2001 [PubMed 11497433]).[supplied by OMIM Jan 2009]

Gene Symbol:   RPP21
Gene Name:   ribonuclease P/MRP 21kDa subunit
Aliases:   C6orf135, CAT60, FLJ22638
RefSeq:   NC_000006.11 NT_007592.15 NT_113891.2 NT_167244.1 NT_167245.1 NT_167246.1 NT_167248.1 NT_167249.1
Ensembl:   ENSG00000241370
Entrez:   79897
Chromosome Mapping:   6p22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.300000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download