PrimePCR™ SYBR® Green Assay: ZBTB22, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040197

List Price:    $174.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037474
Assay Design:   exonic
Chromosome Location:   6:33284477-33284592question
Amplicon Length:   86
Splice Variants Targeted:   ENST00000416097 ENST00000454866 ENST00000548019 ENST00000383196 ENST00000426244 ENST00000552144 ENST00000310999 ENST00000430466 ENST00000431845 ENST00000441117 ENST00000418724 ENST00000418540 ENST00000448500 ENST00000547592 ENST00000361226 ENST00000455600 ENST00000525566 ENST00000503506 ENST00000526691 ENST00000526390 ENST00000429002 ENST00000388854 ENST00000378070 ENST00000397736 ENST00000427956 ENST00000354940 ENST00000548484 ENST00000436352 ENST00000445320

Gene Information

Description Not Available

Gene Symbol:   ZBTB22
Gene Name:   zinc finger and BTB domain containing 22
Aliases:   BING1, ZBTB22A, ZNF297, ZNF297A, fru, fruitless
RefSeq:   NC_000006.11 NG_009876.1 NT_007592.15 NT_113891.2 NT_167245.1 NT_167247.1 NT_167248.1
Ensembl:   ENSG00000236104
Entrez:   9278
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.990000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download