PrimePCR™ SYBR® Green Assay: TCEB3CL, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040191

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TCEB3CL, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TCEB3CL, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037468
Assay Design:   exonic
Chromosome Location:   18:44549863-44549994question
Amplicon Length:   102
Splice Variants Targeted:   ENST00000295148 ENST00000406895 ENST00000557649 ENST00000410013 ENST00000591973 ENST00000451265 ENST00000330682 ENST00000332567 ENST00000252050 ENST00000372647 ENST00000354495 ENST00000382203 ENST00000382194 ENST00000349721 ENST00000357248 ENST00000307659 ENST00000425103 ENST00000377897 ENST00000294179 ENST00000394690 ENST00000541317

Gene Information

Description Not Available

Gene Symbol:   TCEB3CL
Gene Name:   transcription elongation factor B polypeptide 3C-like
Aliases:   HsT828, MGC119353
RefSeq:   NC_000018.9 NT_010966.14
Ensembl:   ENSG00000234298
Entrez:   728929
Chromosome Mapping:   18q21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.980000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download