PrimePCR™ SYBR® Green Assay: TRIM26, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040189

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TRIM26, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TRIM26, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037466
Assay Design:   exonic
Chromosome Location:   6:30166517-30166665question
Amplicon Length:   119
Splice Variants Targeted:   ENST00000447942 ENST00000415789 ENST00000423901 ENST00000427535 ENST00000445259 ENST00000422349 ENST00000425523 ENST00000439094 ENST00000456770 ENST00000422802 ENST00000453442 ENST00000440149 ENST00000383607 ENST00000327357 ENST00000396558 ENST00000412755 ENST00000419171 ENST00000427612 ENST00000433314 ENST00000447711 ENST00000456093 ENST00000438785 ENST00000433836 ENST00000429583 ENST00000425831 ENST00000432326 ENST00000438384 ENST00000449136 ENST00000419401 ENST00000445457 ENST00000437089 ENST

Gene Information

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains a RING a B-box type 1 and a B-box type 2 and a coiled-coil region. The protein localizes to cytoplasmic bodies. Although the function of the protein is unknown the RING domain suggests that the protein may have DNA-binding activity. The gene localizes to the major histocompatibility complex (MHC) class I region on chromosome 6. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq Jun 2011]

Gene Symbol:   TRIM26
Gene Name:   tripartite motif containing 26
Aliases:   AFP, RNF95, ZNF173
RefSeq:   NC_000006.11 NT_007592.15 NT_113891.2 NT_167244.1 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1 NT_167249.1
Ensembl:   ENSG00000234127
Entrez:   7726
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.040000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download