PrimePCR™ SYBR® Green Assay: FAM203B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040175

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: FAM203B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: FAM203B, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037452
Assay Design:   exonic
Chromosome Location:   8:145440303-145440415question
Amplicon Length:   83
Splice Variants Targeted:   ENST00000407595 ENST00000265742 ENST00000347708 ENST00000445970 ENST00000281806 ENST00000377406 ENST00000527931 ENST00000293350 ENST00000455361 ENST00000549706 ENST00000553030 ENST00000042931 ENST00000318636 ENST00000371806 ENST00000304166 ENST00000284476 ENST00000374530 ENST00000302798 ENST00000434623 ENST00000374525 ENST00000480388 ENST00000259318 ENST00000555236 ENST00000510514 ENST00000359548 ENST00000434632 ENST00000377137

Gene Information

Description Not Available

Gene Symbol:   FAM203B
Gene Name:   family with sequence similarity 203, member B
Aliases:   BRP16L, C8orf30B
RefSeq:   NC_000008.10 NT_037704.5
Ensembl:   ENSG00000230567
Entrez:   728071
Chromosome Mapping:   8q24.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.310000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download