PrimePCR™ SYBR® Green Assay: TSPY8, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040170

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037447
Assay Design:   exonic
Chromosome Location:   Y:9195543-9195674question
Amplicon Length:   102
Splice Variants Targeted:   ENST00000320701 ENST00000383042 ENST00000426950 ENST00000383008 ENST00000287721 ENST00000383000 ENST00000383005 ENST00000330628 ENST00000537415 ENST00000457222 ENST00000424594 ENST00000440483 ENST00000451548 ENST00000423647 ENST00000450145 ENST00000423213 ENST00000430032 ENST00000440215 ENST00000428845 ENST00000444056 ENST00000421178 ENST00000358127 ENST00000311303 ENST00000448542 ENST00000416054 ENST00000391967 ENST00000367313 ENST00000366663 ENST00000470540 ENST00000427697

Gene Information

Description Not Available

Gene Symbol:   TSPY8
Gene Name:   testis specific protein, Y-linked 8
Aliases:   Not Available
RefSeq:   NC_000024.9 NT_086998.1
Ensembl:   ENSG00000229549
Entrez:   728403
UniGene:   Hs.740264
Chromosome Mapping:   Yp11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.390000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download