PrimePCR™ SYBR® Green Assay: PNMA6A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040143

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037420
Assay Design:   exonic
Chromosome Location:   X:152339795-152339943question
Amplicon Length:   119
Splice Variants Targeted:   ENST00000309117 ENST00000288304 ENST00000404891 ENST00000472232 ENST00000525144 ENST00000529097 ENST00000525704 ENST00000468418 ENST00000361582 ENST00000463306 ENST00000306117 ENST00000537075 ENST00000252997 ENST00000176763 ENST00000261799 ENST00000560540 ENST00000290894 ENST00000560734 ENST00000560413 ENST00000458022 ENST00000318390 ENST00000229335 ENST00000543081 ENST00000405995 ENST00000320547 ENST00000357304 ENST00000392108 ENST00000421798 ENST00000453825 ENST00000535416 ENST00000538162 ENST

Gene Information

Description Not Available

Gene Symbol:   PNMA6A
Gene Name:   paraneoplastic antigen like 6A
Aliases:   MA6, MGC15827, PNMA6
RefSeq:   NC_000023.10 NG_016372.1 NT_167198.1
Ensembl:   ENSG00000224201
Entrez:   84968
UniGene:   Hs.721963
Chromosome Mapping:   Xq28

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 34.670000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download